Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver

Nobuaki Suzuki, Hirotaka Uefuji, Takashi Nishikawa, Yukio Mukai, Atsushi Yamashita, Masahira Hattori, Naotake Ogasawara, Takeshi Bamba, Ei ichiro Fukusaki, Akio Kobayashi, Yoshiyuki Ogata, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Yoshihisa Nakazawa

研究成果: Article

19 引用 (Scopus)

抄録

Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) representing 10,520 unigenes made up of 4,302 contigs and 6,218 singletons. Homologues of genes coding for rubber particle membrane proteins that participate in the synthesis of high-molecular poly-isoprene in latex were isolated, as well as those encoding known major latex proteins (MLPs). MLPs extensively shared ESTs, indicating their abundant expression during trans-polyisoprene rubber biosynthesis. The six mevalonate pathway genes which are implicated in the synthesis of isopentenyl diphosphate (IPP), a starting material of poly-isoprene biosynthesis, were isolated, and their role in IPP biosynthesis was confirmed by functional complementation of suitable yeast mutants. Genes encoding five full-length trans-isoprenyl diphosphate synthases were also isolated, and two among those synthesized farnesyl diphosphate from IPP and dimethylallyl diphosphate, an assumed intermediate of rubber biosynthesis. This study should provide a valuable resource for further studies of rubber synthesis in E. ulmoides.

元の言語English
ページ(範囲)1405-1417
ページ数13
ジャーナルPlanta
236
発行部数5
DOI
出版物ステータスPublished - 2012 10
外部発表Yes

Fingerprint

Eucommiaceae
Eucommia ulmoides
Expressed Sequence Tags
Rubber
expressed sequence tags
rubber
Latex
latex
biosynthesis
Genes
Mevalonic Acid
unigenes
stems
synthesis
genes
Cotyledon
Diphosphates
Gene Library
woody plants
cDNA libraries

ASJC Scopus subject areas

  • Plant Science
  • Genetics

これを引用

Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver. / Suzuki, Nobuaki; Uefuji, Hirotaka; Nishikawa, Takashi; Mukai, Yukio; Yamashita, Atsushi; Hattori, Masahira; Ogasawara, Naotake; Bamba, Takeshi; Fukusaki, Ei ichiro; Kobayashi, Akio; Ogata, Yoshiyuki; Sakurai, Nozomu; Suzuki, Hideyuki; Shibata, Daisuke; Nakazawa, Yoshihisa.

:: Planta, 巻 236, 番号 5, 10.2012, p. 1405-1417.

研究成果: Article

Suzuki, N, Uefuji, H, Nishikawa, T, Mukai, Y, Yamashita, A, Hattori, M, Ogasawara, N, Bamba, T, Fukusaki, EI, Kobayashi, A, Ogata, Y, Sakurai, N, Suzuki, H, Shibata, D & Nakazawa, Y 2012, 'Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver', Planta, 巻. 236, 番号 5, pp. 1405-1417. https://doi.org/10.1007/s00425-012-1679-x
Suzuki, Nobuaki ; Uefuji, Hirotaka ; Nishikawa, Takashi ; Mukai, Yukio ; Yamashita, Atsushi ; Hattori, Masahira ; Ogasawara, Naotake ; Bamba, Takeshi ; Fukusaki, Ei ichiro ; Kobayashi, Akio ; Ogata, Yoshiyuki ; Sakurai, Nozomu ; Suzuki, Hideyuki ; Shibata, Daisuke ; Nakazawa, Yoshihisa. / Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver. :: Planta. 2012 ; 巻 236, 番号 5. pp. 1405-1417.
@article{ce4a8b1d1ef64b33bb441669adbe61e9,
title = "Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver",
abstract = "Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) representing 10,520 unigenes made up of 4,302 contigs and 6,218 singletons. Homologues of genes coding for rubber particle membrane proteins that participate in the synthesis of high-molecular poly-isoprene in latex were isolated, as well as those encoding known major latex proteins (MLPs). MLPs extensively shared ESTs, indicating their abundant expression during trans-polyisoprene rubber biosynthesis. The six mevalonate pathway genes which are implicated in the synthesis of isopentenyl diphosphate (IPP), a starting material of poly-isoprene biosynthesis, were isolated, and their role in IPP biosynthesis was confirmed by functional complementation of suitable yeast mutants. Genes encoding five full-length trans-isoprenyl diphosphate synthases were also isolated, and two among those synthesized farnesyl diphosphate from IPP and dimethylallyl diphosphate, an assumed intermediate of rubber biosynthesis. This study should provide a valuable resource for further studies of rubber synthesis in E. ulmoides.",
keywords = "EST, Eucommia ulmoides Oliv., Hardy rubber tree, Isoprenoid, Prenyltransferase, Trans-polyisoprene",
author = "Nobuaki Suzuki and Hirotaka Uefuji and Takashi Nishikawa and Yukio Mukai and Atsushi Yamashita and Masahira Hattori and Naotake Ogasawara and Takeshi Bamba and Fukusaki, {Ei ichiro} and Akio Kobayashi and Yoshiyuki Ogata and Nozomu Sakurai and Hideyuki Suzuki and Daisuke Shibata and Yoshihisa Nakazawa",
year = "2012",
month = "10",
doi = "10.1007/s00425-012-1679-x",
language = "English",
volume = "236",
pages = "1405--1417",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver

AU - Suzuki, Nobuaki

AU - Uefuji, Hirotaka

AU - Nishikawa, Takashi

AU - Mukai, Yukio

AU - Yamashita, Atsushi

AU - Hattori, Masahira

AU - Ogasawara, Naotake

AU - Bamba, Takeshi

AU - Fukusaki, Ei ichiro

AU - Kobayashi, Akio

AU - Ogata, Yoshiyuki

AU - Sakurai, Nozomu

AU - Suzuki, Hideyuki

AU - Shibata, Daisuke

AU - Nakazawa, Yoshihisa

PY - 2012/10

Y1 - 2012/10

N2 - Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) representing 10,520 unigenes made up of 4,302 contigs and 6,218 singletons. Homologues of genes coding for rubber particle membrane proteins that participate in the synthesis of high-molecular poly-isoprene in latex were isolated, as well as those encoding known major latex proteins (MLPs). MLPs extensively shared ESTs, indicating their abundant expression during trans-polyisoprene rubber biosynthesis. The six mevalonate pathway genes which are implicated in the synthesis of isopentenyl diphosphate (IPP), a starting material of poly-isoprene biosynthesis, were isolated, and their role in IPP biosynthesis was confirmed by functional complementation of suitable yeast mutants. Genes encoding five full-length trans-isoprenyl diphosphate synthases were also isolated, and two among those synthesized farnesyl diphosphate from IPP and dimethylallyl diphosphate, an assumed intermediate of rubber biosynthesis. This study should provide a valuable resource for further studies of rubber synthesis in E. ulmoides.

AB - Eucommia ulmoides Oliver is one of a few woody plants capable of producing abundant quantities of trans-polyisoprene rubber in their leaves, barks, and seed coats. One cDNA library each was constructed from its outer stem tissue and inner stem tissue. They comprised a total of 27,752 expressed sequence tags (ESTs) representing 10,520 unigenes made up of 4,302 contigs and 6,218 singletons. Homologues of genes coding for rubber particle membrane proteins that participate in the synthesis of high-molecular poly-isoprene in latex were isolated, as well as those encoding known major latex proteins (MLPs). MLPs extensively shared ESTs, indicating their abundant expression during trans-polyisoprene rubber biosynthesis. The six mevalonate pathway genes which are implicated in the synthesis of isopentenyl diphosphate (IPP), a starting material of poly-isoprene biosynthesis, were isolated, and their role in IPP biosynthesis was confirmed by functional complementation of suitable yeast mutants. Genes encoding five full-length trans-isoprenyl diphosphate synthases were also isolated, and two among those synthesized farnesyl diphosphate from IPP and dimethylallyl diphosphate, an assumed intermediate of rubber biosynthesis. This study should provide a valuable resource for further studies of rubber synthesis in E. ulmoides.

KW - EST

KW - Eucommia ulmoides Oliv.

KW - Hardy rubber tree

KW - Isoprenoid

KW - Prenyltransferase

KW - Trans-polyisoprene

UR - http://www.scopus.com/inward/record.url?scp=84868123724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868123724&partnerID=8YFLogxK

U2 - 10.1007/s00425-012-1679-x

DO - 10.1007/s00425-012-1679-x

M3 - Article

C2 - 22729820

AN - SCOPUS:84868123724

VL - 236

SP - 1405

EP - 1417

JO - Planta

JF - Planta

SN - 0032-0935

IS - 5

ER -