Construction of an electrochemical probe for on chip type flow immunoassay

Mina Okochi, Hiroko Ohta, Tomoyuki Taguchi, Hiroyuki Ohta, Tadashi Matsunaga

研究成果: Article

12 引用 (Scopus)

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Miniaturized immunosensors, that combine the analytical power of microfluidic devices with the high specificity of biomolecules, have been intensively studied for clinical analyses, environmental immunoassays and biochemical studies. Protein A has the ability to bind Fc-binding site of immunoglobulin G (IgG), and can be used as the detection probe in immunoassays. Ferrocenecarboaldehyde (Fc-CHO) was labeled to protein A as an electrochemical probe for on chip type amperometric flow immunoassay. When labeling Fc-CHO onto protein A via formation of Schiff-base and its reduction, mean number of nine Fc-CHO labeled to individual protein A molecules. Protein A labeled with Fc-CHO exhibited reversible redox properties of ferrocene in cyclic voltammograms and its peak currents increased with number of labeled ferrocenes. The current response of protein A labeled with Fc-CHO well correlated with its concentration when applying 390 V versus Ag/AgCl. Protein A labeled with Fc-CHO showed sufficient electrochemical properties that are useful in a miniaturized electrochemical immunoassays.

元の言語English
ページ(範囲)952-955
ページ数4
ジャーナルElectrochimica Acta
51
発行部数5
DOI
出版物ステータスPublished - 2005 11 10
外部発表Yes

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ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

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