Construction of carotenoid biosynthetic pathways using squalene synthase

Maiko Furubayashi, Ling Li, Akinori Katabami, Kyoichi Saito, Daisuke Umeno*

*この研究の対応する著者

研究成果: Article査読

23 被引用数 (Scopus)

抄録

The first committed steps of steroid/hopanoid pathways involve squalene synthase (SQS). Here, we report the Escherichia coli production of diaponeurosporene and diapolycopene, yellow C30 carotenoid pigments, by expressing human SQS and Staphylococcus aureus dehydrosqualene (C 30 carotenoid) desaturase (CrtN). We suggest that the carotenoid pigments are synthesized mainly via the desaturation of squalene rather than the direct synthesis of dehydrosqualene through the non-reductive condensation of prenyl diphosphate precursors, indicating the possible existence of a "squalene route" and a "lycopersene route" for C 30 and C40 carotenoids, respectively. Additionally, this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs.

本文言語English
ページ(範囲)436-442
ページ数7
ジャーナルFEBS Letters
588
3
DOI
出版ステータスPublished - 2014 1月 31
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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