Construction of electrochemical flow immunoassay system using capillary columns and ferrocene conjugated immunoglobulin G for detection of human chorionic gonadotrophin

Tae Kyu Lim, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

53 被引用数 (Scopus)

抄録

In this paper is reported a miniaturized flow immunoassay system. Ferrocenecarboxylic acid (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared, and used as a novel analytical reagent. The system consists of the immunoreaction section, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. Antibody-antigen complexes were separated from their free conjugate on the basis of differences in isoelectric point (pI) using a cation exchange capillary column. The assay yielded a linear relationship between signal and HCG concentration in the range 0-2000 mIU/ml. This simple technique enables the assay of HCG within 2 min. The cation exchange capillary column was regenerated by occasional elution with malonate buffer (pH 6.0) containing 0.5 M NaCl, to remove free conjugate. Free conjugate recovered in this manner could be reused up to eight times without significant decreases in the sensitivity of the immunoassay. This electrochemical flow immunoassay requires only minute quantities of serum and generates highly reproducible results.

本文言語English
ページ(範囲)1063-1069
ページ数7
ジャーナルBiosensors and Bioelectronics
16
9-12
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 電気化学

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