Contributions of phosphate to DNA adsorption/desorption behaviors on aminosilane-modified magnetic nanoparticles

Tsuyoshi Tanaka, Ririko Sakai, Ryosuke Kobayashi, Keiichi Hatakeyama, Tadashi Matsunaga

研究成果: Article査読

83 被引用数 (Scopus)

抄録

The adsorption and desorption behaviors of DNA on aminosilane-modified magnetic nanoparticles were investigated by altering both type of anions and solvation state to achieve eficient recovery of DNA useful for subsequent polymerase chain reaction (PCR) analysis. The effects of multiple anions in accordance with the Hofmeister ion series were determined to clarify the contribution of phosphate ions on the effective desorption of DNA from aminosilane surfaces. Efficient DNA desorption (85% recovery) occurred in the presence of 1 M phosphate buffer, however, little DNA desorption was observed using any other anions. This phenomenon indicates that desorption originated from the replacement of DNA by phosphate ions. Furthermore, the adsorption and desorption were significantly affected by the addition of both protic and aprotic solvents. Efficient recovery of adsorbed DNA was attained using deoxynucleotide triphosphates (dNTPs) in place of phosphate buffer and was suitable for subsequent PCR analyses. Therefore, the DNA adsorption/desorption process proposed in this study will be a promising, novel approach for DNA purification with a high recovery ratio that is suitable for subsequent enzymatic reactions, such as PCR or restriction enzyme digestion.

本文言語English
ページ(範囲)2956-2961
ページ数6
ジャーナルLangmuir
25
5
DOI
出版ステータスPublished - 2009 3 3
外部発表はい

ASJC Scopus subject areas

  • 電気化学
  • 凝縮系物理学
  • 表面および界面
  • 材料科学(全般)
  • 分光学

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