Controllable three-dimensional sheath flow with a wide range reynolds number and its application for efficient cell sorting

R. Sekine, T. Sakurai, D. H. Yoon, R. Iizuka, T. Sekiguchi, T. Funatsu, S. Shoji

研究成果: Conference contribution

抄録

We have developed on-chip sorting systems using sol-gel transition of the thermo-reversible gelation polymer (TGP) as carrier flow controlled by the focused infrared (IR) laser [1]. For efficient sorting, we achieved size and position control of sample core flow in three-dimensional (3D) sheath flow. Stable control was realized under wide range of Reynolds number (Re) from 0.1 to 115 by optimizing channel structures of sample and carriers inlets. We applied this device for sorting system of yeast cells expressing green fluorescent protein (GFP). The yeast cells were successfully separated with accuracy of more than 82.1% and purity of more than 97.5%.

本文言語English
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ1903-1905
ページ数3
出版ステータスPublished - 2011 12月 1
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
継続期間: 2011 10月 22011 10月 6

出版物シリーズ

名前15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
3

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
国/地域United States
CitySeattle, WA
Period11/10/211/10/6

ASJC Scopus subject areas

  • 制御およびシステム工学

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