Controlling the direction of kinesin-driven microtubule movements along microlithographic tracks

Yuichi Hiratsuka, Tetsuya Tada, Kazuhiro Oiwa, Toshihiko Kanayama, Taro Q.P. Uyeda*

*この研究の対応する著者

研究成果: Article査読

324 被引用数 (Scopus)

抄録

Motor proteins are able to move protein filaments in vitro. However, useful work cannot be extracted from the existing in vitro systems because filament motions are in random directions on two-dimensional surfaces. We succeeded in restricting kinesin-driven movements of microtubules along linear tracks by using micrometer-scaled grooves lithographically fabricated on glass surfaces. We also accomplished the extraction of unidirectional movement from the bidirectional movements along the linear tracks by adding arrowhead patterns on the tracks. These "rectifiers" enabled us to construct microminiturized circulators in which populations of microtubules rotated in one direction, and to actively transport microtubules between two pools connected by arrowheaded tracks in the fields of micrometer scales.

本文言語English
ページ(範囲)1555-1561
ページ数7
ジャーナルBiophysical Journal
81
3
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 生物理学

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