Convenient method to purify hemoglobin

Hiromi Sakai, Shinji Takeoka, Hiroyuki Nishide, Eishun Tsuchida

研究成果: Article査読

8 被引用数 (Scopus)

抄録

A convenient method to purify Hb solution from outdated RBC has been established for the starting material of Hb-based blood substitutes. To prevent MetHb formation during the procedure, Hb in RBC was carbonylated in advance. Then RBC was mixed with organic solvent for hemolysis and centrifuged for removal of stroma. The resulting SFHb solution was heated at 60C and generated precipitates were removed out by centrifugation. The purity of Hb (25 g/dl) was confirmed by SDS-PAGE. IEF and oxygen binding property of the Hb solution also guaranteed its purity and no denaturation of Hb. This method is applicable to large scale production of the purified Hb for the starting material of Hb-based blood substitutes.

本文言語English
ページ(範囲)651-656
ページ数6
ジャーナルArtificial Cells, Blood Substitutes, and Biotechnology
22
3
DOI
出版ステータスPublished - 1994 1月

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生体医工学

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