Cooperative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells

Hideki Nakamura, Hiroko Bannai, Takafumi Inoue, Takayuki Michikawa, Masaki Sano, Katsuhiko Mikoshiba

    研究成果: Article

    5 引用 (Scopus)

    抄録

    Ca2+ microdomains or locally restricted Ca2+ increases in the cell have recently been reported to regulate many essential physiological events. Ca2+ increases through the inositol 1,4,5-trisphosphate(IP3)receptor(IP3R)/Ca2+ release channels contribute to the formation of a class of such Ca2+ microdomains, which were often observed and referred to as Ca2+ puffs in their isolated states. In this report, we visualized IP3-evoked Ca2+ microdomains in histamine-stimulated intact HeLa cells using a total internal reflection fluorescence microscope, and quantitatively characterized the spatial profile by fitting recorded images to a two-dimensional Gaussian distribution. Ca2+ concentration profiles were marginally spatially anisotropic, with the size increasing linearly even after the amplitude began to decline. We found the event centroid drifted with an apparent diffusion coefficient of 4.20 ± 0.50 μm2/s, which is significantly larger than those estimated for IP3Rs. The sites of maximal Ca2+ increase, rather than initiation or termination sites, were detected repeatedly at the same location. These results indicate that Ca2+ microdomains in intact HeLa cell are generated from spatially distributed multiple IP3R clusters or Ca2+ puff sites, rather than a single IP3R cluster reported in cells loaded with Ca 2+ buffers.

    元の言語English
    ページ(範囲)24563-24572
    ページ数10
    ジャーナルJournal of Biological Chemistry
    287
    発行部数29
    DOI
    出版物ステータスPublished - 2012 7 13

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    Inositol 1,4,5-Trisphosphate Receptors
    Calcium Signaling
    HeLa Cells
    Calcium
    Inositol 1,4,5-Trisphosphate
    Normal Distribution
    Gaussian distribution
    Histamine
    Buffers
    Microscopes
    Fluorescence

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    これを引用

    Cooperative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells. / Nakamura, Hideki; Bannai, Hiroko; Inoue, Takafumi; Michikawa, Takayuki; Sano, Masaki; Mikoshiba, Katsuhiko.

    :: Journal of Biological Chemistry, 巻 287, 番号 29, 13.07.2012, p. 24563-24572.

    研究成果: Article

    Nakamura, Hideki ; Bannai, Hiroko ; Inoue, Takafumi ; Michikawa, Takayuki ; Sano, Masaki ; Mikoshiba, Katsuhiko. / Cooperative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells. :: Journal of Biological Chemistry. 2012 ; 巻 287, 番号 29. pp. 24563-24572.
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    AU - Sano, Masaki

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