Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses

Chihiro Ikeda, Kazuo Taku, Tsumugi Miyazaki, Rikako Shirai, Richard S. Nelson, Hiroshi Nyunoya, Yasuhiko Matsushita, Nobumitsu Sasaki*

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

The tobacco virus resistance gene N contains four introns. Transient expression of transcripts from an N transgene containing these introns and driven by the native promoter in the presence of the elicitor of tobacco mosaic virus resulted in its increased expression. The requirement of the native promoter, the elicitor, or the individual introns for enhanced expression of N has not been fully studied. Here, we determined that 35S promoter-driven N transcript expression could be enhanced in the presence of the four introns regardless of the co-expression of the virus elicitor in tobacco. Function analyses using a series of N transgenes with different combination of introns revealed that the presence of intron 1 more so than intron 2 allowed higher accumulation of premature and mature N transcripts; however, both introns were important for not only enhanced gene expression but also for induction of cell death in tobacco and induced local resistance to spread of virus in Nicotiana benthamiana. Our findings indicate that introns 1 and 2 cooperatively contribute to N expression and virus resistance.

本文言語English
論文番号15424
ジャーナルScientific reports
11
1
DOI
出版ステータスPublished - 2021 12月

ASJC Scopus subject areas

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