Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles

Kazuhito Tomizawa*, Satoshi Sunada, Yun Fei Lu, Yoshiya Oda, Masahiro Kinuta, Toshio Ohshima, Taro Saito, Fan Yan Wei, Masayuki Matsushita, Sheng Tian Li, Kimiko Tsutsui, Shin Ichi Hisanaga, Katsuhiko Mikoshiba, Kohji Takei, Hideki Matsui

*この研究の対応する著者

研究成果: Article査読

158 被引用数 (Scopus)

抄録

It has been thought that clathrin-mediated endocytosis is regulated by phosphorylation and dephosphorylation of many endocytic proteins, including amphiphysin I and dynamin I. Here, we show that Cdk5/p35-dependent cophosphorylation of amphiphysin I and dynamin I plays a critical role in such processes. Cdk5 inhibitors enhanced the electric stimulation-induced endocytosis in hippocampal neurons, and the endocytosis was also enhanced in the neurons of p35-deficient mice. Cdk5 phosphorylated the proline-rich domain of both amphiphysin I and dynamin I in vitro and in vivo. Cdk5-dependent phosphorylation of amphiphysin I inhibited the association with β-adaptin. Furthermore, the phosphorylation of dynamin I blocked its binding to amphiphysin I. The phosphorylation of each protein reduced the copolymerization into a ring formation in a cell-free system. Moreover, the phosphorylation of both proteins completely disrupted the copolymerization into a ring formation. Finally, phosphorylation of both proteins was undetectable in p35-deficient mice.

本文言語English
ページ(範囲)813-824
ページ数12
ジャーナルJournal of Cell Biology
163
4
DOI
出版ステータスPublished - 2003 11 24
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

フィンガープリント

「Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル