Correlation between cell attachment areas after 2h of culture and osteogenic differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface properties

Kan Cheng, Motohiro Hirose*, Xiupeng Wang, Yu Sogo, Atsushi Yamazaki, Atsuo Ito

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The initial attachment of mesenchymal stem cells (MSCs) to substrates and osteogenic differentiation are supported by culture on a hydroxyapatite substrate. Cell attachment areas of rat MSCs after 2. h of culture on hydroxyapatite substrates with various microstructures and the osteogenic differentiation activity thereafter were measured. The perceived outcome was that, after 2. h of culture, rat MSCs with a small attachment area would have a high osteogenic differentiation activity, whereas those with a large attachment area would have a low osteogenic differentiation activity. Furthermore, rat MSCs with a small attachment area had many cytoplasmic processes, while those with a large attachment area revealed clear stress fibers and focal contacts. These results suggest that cell attachment area of rat MSCs after 2. h of culture has a strong effect on the osteogenic differentiation of rat MSCs. Thus, the measurement of cell attachment area after 2. h of culture could become valuable for estimating the osteogenic differentiation activity of rat MSCs thereafter.

本文言語English
ページ(範囲)156-160
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
430
1
DOI
出版ステータスPublished - 2013 1 4

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Correlation between cell attachment areas after 2h of culture and osteogenic differentiation activity of rat mesenchymal stem cells on hydroxyapatite substrates with various surface properties」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル