CpG methylation of the CENP-B box reduces human CENP-B binding

Yoshinori Tanaka, Hitoshi Kurumizaka*, Shigeyuki Yokoyama

*この研究の対応する著者

    研究成果: Article査読

    22 被引用数 (Scopus)

    抄録

    In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.

    本文言語English
    ページ(範囲)282-289
    ページ数8
    ジャーナルFEBS Journal
    272
    1
    DOI
    出版ステータスPublished - 2005 1

    ASJC Scopus subject areas

    • 生化学

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