Creating Biological Membranes on the Micron Scale: Forming Patterned Lipid Bilayers Using a Polymer Lift-Off Technique

R. N. Orth*, J. Kameoka, W. R. Zipfel, B. Ilic, W. W. Webb, T. G. Clark, H. G. Craighead

*この研究の対応する著者

研究成果: Article査読

78 被引用数 (Scopus)

抄録

We present a new method for creating patches of fluid lipid bilayers with conjugated biotin and other compounds down to 1 μm resolution using a photolithographically patterned polymer lift-off technique. The patterns are realized as the polymer is mechanically peeled away in one contiguous piece in solution. The functionality of these surfaces is verified with binding of antibodies and avidin on these uniform micron-scale platforms. The biomaterial patches, measuring 1 μm-76 μm on edge, provide a synthetic biological substrate for biochemical analysis that is ∼100× smaller in width than commercial printing technologies. 100 nm unilamellar lipid vesicles spread to form a supported fluid lipid bilayer on oxidized silicon surface as confirmed by fluorescence photobleaching recovery. Fluorescence photobleaching recovery measurements of Dil (1,1′-dioctadecyl-3,3,3′,3′ -tetramethylindocarbocyanine perchlorate (DiIC18(3))) stained bilayer patches yielded an average diffusion coefficient of 7.54 ± 1.25 μm2 s-1, equal to or slightly faster than typically found in Dil stained cells. This diffusion rate is ∼3× faster than previous values for bilayers on glass. This method provides a new means to form functionalized fluid lipid bilayers as micron-scale platforms to immobilize biomaterials, capture antibodies and biotinylated reagents from solution, and form antigenic stimuli for cell stimulation.

本文言語English
ページ(範囲)3066-3073
ページ数8
ジャーナルBiophysical Journal
85
5
DOI
出版ステータスPublished - 2003 11月
外部発表はい

ASJC Scopus subject areas

  • 生物理学

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