Creation of novel technologies for extracellular protein production toward the development of bacillus subtilis genome factories

Katsutoshi Ara, Kenji Manabe, Shenghao Liu, Yasushi Kageyama, Tadahiro Ozawa, Masatoshi Tohata, Keiji Endo, Kazuhisa Sawada, Nozomu Shibata, Akihito Kawahara, Kazuhiro Saito, Hiroshi Kodama, Yoshiharu Kimura, Katsuya Ozaki, Yoshinori Takema, Hiroshi Kakeshita, Kouji Nakamura, Kunio Yamane, Takeko Kodama, Junichi SekiguchiTakuya Morimoto, Ryosuke Kadoya, Shigehiko Kanaya, Yasutaro Fujita, Fujio Kawamura, Naotake Ogasawara

研究成果: Chapter


Bacillus subtilis has been widely used for the industrial production of useful proteins because of its high protein secretion ability and safety. We focused on genome reduction as a new concept for enhancing production of recombinant enzymes in B. subtilis cells based on detailed analysis of the genome mechanism. First, we reported that a novel B. subtilis strain, MGB874, depleted 20.7 % of the genomic sequence of the wild type by rationally designed deletions to create simplified cells for protein production. When compared with wild-type cells, the productivity of cellulase and protease from transformed plasmids harboring the corresponding genes was markedly enhanced. These results indicate that a bacterial factory specializing in the production of substances can be constructed by deleting the genomic regions unimportant for growth and substance production from B. subtilis. Second, deletion of the rocDEF-rocR region, which is involved in arginine degradation, was found to contribute to the improvement of enzyme production in strain MGB874. The present study indicated that our results demonstrated the effectiveness of a synthetic genomic approach with reduction of genome size to generate novel and useful bacteria for industrial uses. Furthermore, the design of the changes in the transcriptional regulatory network of the nitrogen metabolic pathway in B. subtilis cells could facilitate the generation of improved industrial protein production.

ホスト出版物のタイトルMicrobial Production
ホスト出版物のサブタイトルFrom Genome Design to Cell Engineering
出版社Springer Japan
ISBN(印刷版)4431546065, 9784431546061
出版ステータスPublished - 2013 11 1

ASJC Scopus subject areas

  • 免疫学および微生物学(全般)
  • 生化学、遺伝学、分子生物学(全般)


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