Crystal structure of the nucleosome containing ultraviolet light-induced cyclobutane pyrimidine dimer

Naoki Horikoshi, Hiroaki Tachiwana, Wataru Kagawa, Akihisa Osakabe, Syota Matsumoto, Shigenori Iwai, Kaoru Sugasawa, Hitoshi Kurumizaka

研究成果: Article

10 引用 (Scopus)

抜粋

The cyclobutane pyrimidine dimer (CPD) is induced in genomic DNA by ultraviolet (UV) light. In mammals, this photolesion is primarily induced within nucleosomal DNA, and repaired exclusively by the nucleotide excision repair (NER) pathway. However, the mechanism by which the CPD is accommodated within the nucleosome has remained unknown. We now report the crystal structure of a nucleosome containing CPDs. In the nucleosome, the CPD induces only limited local backbone distortion, and the affected bases are accommodated within the duplex. Interestingly, one of the affected thymine bases is located within 3.0 Å from the undamaged complementary adenine base, suggesting the formation of complementary hydrogen bonds in the nucleosome. We also found that UV-DDB, which binds the CPD at the initial stage of the NER pathway, also efficiently binds to the nucleosomal CPD. These results provide important structural and biochemical information for understanding how the CPD is accommodated and recognized in chromatin.

元の言語English
ページ(範囲)117-122
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
471
発行部数1
DOI
出版物ステータスPublished - 2016 2 26

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

フィンガープリント Crystal structure of the nucleosome containing ultraviolet light-induced cyclobutane pyrimidine dimer' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Horikoshi, N., Tachiwana, H., Kagawa, W., Osakabe, A., Matsumoto, S., Iwai, S., Sugasawa, K., & Kurumizaka, H. (2016). Crystal structure of the nucleosome containing ultraviolet light-induced cyclobutane pyrimidine dimer. Biochemical and Biophysical Research Communications, 471(1), 117-122. https://doi.org/10.1016/j.bbrc.2016.01.170