Crystal structure of the overlapping dinucleosome composed of hexasome and octasome

Daiki Kato; Akihisa Osakabe; Yasuhiro Arimura; Yuka Mizukami; Naoki Horikoshi; Kazumi Saikusa; Satoko Akashi; Yoshifumi Nishimura; Sam Yong Park; Jumpei Nogami; Kazumitsu Maehara; Yasuyuki Ohkawa; Atsushi Matsumoto; Hidetoshi Kono; Rintaro Inoue; Masaaki Sugiyama; Hitoshi Kurumizaka

doi:10.1126/science.aak9867

Crystal structure of the overlapping dinucleosome composed of hexasome and octasome

Daiki Kato, Akihisa Osakabe, Yasuhiro Arimura, Yuka Mizukami, Naoki Horikoshi, Kazumi Saikusa, Satoko Akashi, Yoshifumi Nishimura, Sam Yong Park, Jumpei Nogami, Kazumitsu Maehara, Yasuyuki Ohkawa, Atsushi Matsumoto, Hidetoshi Kono, Rintaro Inoue, Masaaki Sugiyama, Hitoshi Kurumizaka^*

^*この研究の対応する著者

理工学術院総合研究所

研究成果: Article › 査読

51 被引用数 (Scopus)

抄録

Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate "overlapping dinucleosome." Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual "hexasome" nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical "octasome" nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.

本文言語	English
論文番号	356
ページ（範囲）	205-208
ページ数	4
ジャーナル	Science
巻	356
号	6334
DOI	https://doi.org/10.1126/science.aak9867
出版ステータス	Published - 2017 4月 14

ASJC Scopus subject areas

医学（全般）
一般

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10.1126/science.aak9867

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Kato, D., Osakabe, A., Arimura, Y., Mizukami, Y., Horikoshi, N., Saikusa, K., Akashi, S., Nishimura, Y., Park, S. Y., Nogami, J., Maehara, K., Ohkawa, Y., Matsumoto, A., Kono, H., Inoue, R., Sugiyama, M., & Kurumizaka, H. (2017). Crystal structure of the overlapping dinucleosome composed of hexasome and octasome. Science, 356(6334), 205-208. [356]. https://doi.org/10.1126/science.aak9867

Kato, D, Osakabe, A, Arimura, Y, Mizukami, Y, Horikoshi, N, Saikusa, K, Akashi, S, Nishimura, Y, Park, SY, Nogami, J, Maehara, K, Ohkawa, Y, Matsumoto, A, Kono, H, Inoue, R, Sugiyama, M & Kurumizaka, H 2017, 'Crystal structure of the overlapping dinucleosome composed of hexasome and octasome', Science, vol. 356, no. 6334, 356, pp. 205-208. https://doi.org/10.1126/science.aak9867

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title = "Crystal structure of the overlapping dinucleosome composed of hexasome and octasome",

abstract = "Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate {"}overlapping dinucleosome.{"} Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual {"}hexasome{"} nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical {"}octasome{"} nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.",

author = "Daiki Kato and Akihisa Osakabe and Yasuhiro Arimura and Yuka Mizukami and Naoki Horikoshi and Kazumi Saikusa and Satoko Akashi and Yoshifumi Nishimura and Park, {Sam Yong} and Jumpei Nogami and Kazumitsu Maehara and Yasuyuki Ohkawa and Atsushi Matsumoto and Hidetoshi Kono and Rintaro Inoue and Masaaki Sugiyama and Hitoshi Kurumizaka",

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AU - Kato, Daiki

AU - Osakabe, Akihisa

AU - Arimura, Yasuhiro

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AU - Horikoshi, Naoki

AU - Saikusa, Kazumi

AU - Akashi, Satoko

AU - Nishimura, Yoshifumi

AU - Park, Sam Yong

AU - Nogami, Jumpei

AU - Maehara, Kazumitsu

AU - Ohkawa, Yasuyuki

AU - Matsumoto, Atsushi

AU - Kono, Hidetoshi

AU - Inoue, Rintaro

AU - Sugiyama, Masaaki

AU - Kurumizaka, Hitoshi

PY - 2017/4/14

Y1 - 2017/4/14

N2 - Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate "overlapping dinucleosome." Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual "hexasome" nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical "octasome" nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.

AB - Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate "overlapping dinucleosome." Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual "hexasome" nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical "octasome" nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.

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Crystal structure of the overlapping dinucleosome composed of hexasome and octasome

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