Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TC domain of tRNA

Kosuke Ito; Hao Qi; Yoshihiro Shimizu; Ryo Murakami; Kin Ichiro Miura; Takuya Ueda; Toshio Uchiumi

doi:10.1107/S1744309111038383

Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TC domain of tRNA

Kosuke Ito^*, Hao Qi, Yoshihiro Shimizu, Ryo Murakami, Kin Ichiro Miura, Takuya Ueda, Toshio Uchiumi

^*この研究の対応する著者

研究成果: Article › 査読

9 被引用数 (Scopus)

抄録

Peptidyl-tRNA hydrolase (Pth) cleaves the ester bond between the peptide and the tRNA of peptidyl-tRNA molecules, which are the product of aborted translation. In the present work, Pth from Escherichia coli was crystallized with the acceptor-TC domain of tRNA using 1,4-butanediol as a precipitant. The crystals belonged to the hexagonal space group P61, with unit-cell parameters a = b = 55.1, c = 413.1 Å, and diffracted X-rays beyond 2.4 Å resolution. The asymmetric unit is expected to contain two complexes of Pth and the acceptor-TC domain of tRNA (V M = 2.8 Å ³ Da ^-1), with a solvent content of 60.8%. The structure is being solved by molecular replacement.

本文言語	English
ページ（範囲）	1566-1569
ページ数	4
ジャーナル	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
巻	67
号	12
DOI	https://doi.org/10.1107/S1744309111038383
出版ステータス	Published - 2011 12月
外部発表	はい

ASJC Scopus subject areas

生物理学
構造生物学
生化学
遺伝学
凝縮系物理学

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10.1107/S1744309111038383

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引用スタイル

Ito, K., Qi, H., Shimizu, Y., Murakami, R., Miura, K. I., Ueda, T., & Uchiumi, T. (2011). Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TC domain of tRNA. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 67(12), 1566-1569. https://doi.org/10.1107/S1744309111038383

Ito, K, Qi, H, Shimizu, Y, Murakami, R, Miura, KI, Ueda, T & Uchiumi, T 2011, 'Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TC domain of tRNA', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 67, no. 12, pp. 1566-1569. https://doi.org/10.1107/S1744309111038383

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