The Athe-0614 protein is a component of the extracellular proteins secreted by the anaerobic, extremely thermophilic and cellulolytic bacterium Caldicellulosiruptor bescii. The recombinant protein was expressed in Escherichia coli, purified to near-homogeneity and crystallized using polyethylene glycol 2000 monomethyl ether as a precipitant. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 48.4, b = 42.2, c = 97.8 Å, β = 96.1°, and diffracted to 2.7 Å resolution using synchrotron radiation.
|ジャーナル||Acta Crystallographica Section F: Structural Biology and Crystallization Communications|
|出版ステータス||Published - 2013 4|
ASJC Scopus subject areas
- Structural Biology
- Condensed Matter Physics