Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics

K. Nakamura, R. Iizuka, T. Yoshida, Y. Hatada, Y. Takaki, S. Nishi, A. Iguchi, Donghyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji, T. Funatsu

研究成果: Conference contribution

抜粋

Environmental microbes are a great source of industrially valuable enzymes with high and unique catalytic activities. However, a vast majority of microbes remain unculturable and thus are not accessible by culture-based methods. Here, we present a rapid and efficient method to screen and identify enzyme-encoding genes from environmental microbes in a culture-independent manner. This method combines activity-based single cell screening using microdroplets and single cell genomics. Using this method, we successfully identified 13 novel β-glucosidase genes from uncultured marine bacteria. This method will facilitate the identification of genes encoding industrially valuable enzymes.

元の言語English
ホスト出版物のタイトルMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版者Chemical and Biological Microsystems Society
ページ513-515
ページ数3
ISBN(電子版)9780979806483
出版物ステータスPublished - 2015
イベント19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
継続期間: 2015 10 252015 10 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Korea, Republic of
Gyeongju
期間15/10/2515/10/29

ASJC Scopus subject areas

  • Control and Systems Engineering

フィンガープリント Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Nakamura, K., Iizuka, R., Yoshida, T., Hatada, Y., Takaki, Y., Nishi, S., Iguchi, A., Yoon, D., Sekiguchi, T., Shoji, S., & Funatsu, T. (2015). Culture-independent method for screening and identifying microbial enzyme-encoding genes using microdroplet-based single cell genomics. : MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 513-515). Chemical and Biological Microsystems Society.