Cup-Shaped superparamagnetic hemispheres for Size-Selective cell filtration

Hyonchol Kim, Hideyuki Terazono, Hiroyuki Takei, Kenji Yasuda

研究成果: Article査読

11 被引用数 (Scopus)

抄録

We propose a new method of size separation of cells exploiting precisely size-controlled hemispherical superparamagnetic microparticles. A three-layered structure of a 2-nm nickel layer inserted between 15-nm silicon dioxide layers was formed on polystyrene cast spheres by vapor deposition. The polystyrene was then removed by burning and the hemispherical superparamagnetic microparticles, "magcups", were obtained. The standard target cells (CCRF-CEM, 12 ± 2 μm) were mixed with a set of different sizes of the fabricated magcups, and we confirmed that the cells were captured in the magcups having cavities larger than 15 μm in diameter, and then gathered by magnetic force. The collected cells were grown in a culture medium without any damage. The results suggest that this method is quick, simple and non-invasive size separation of target cells.

本文言語English
論文番号6362
ジャーナルScientific reports
4
DOI
出版ステータスPublished - 2014
外部発表はい

ASJC Scopus subject areas

  • 一般

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