TY - JOUR
T1 - Curcumin attenuates oxidative stress following downhill running-induced muscle damage
AU - Kawanishi, Noriaki
AU - Kato, Kouki
AU - Takahashi, Masaki
AU - Mizokami, Tsubasa
AU - Otsuka, Yoshihiko
AU - Imaizumi, Atsushi
AU - Shiva, Daisuke
AU - Yano, Hiromi
AU - Suzuki, Katsuhiko
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid for the Global COE “Sport Sciences for the Promotion of Active Life” from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to Professor K. Kanosue).
PY - 2013/11/22
Y1 - 2013/11/22
N2 - Downhill running causes muscle damage, and induces oxidative stress and inflammatory reaction. Recently, it is shown that curcumin possesses anti-oxidant and anti-inflammatory potentials. Interestingly, curcumin reduces inflammatory cytokine concentrations in skeletal muscle after downhill running of mice. However, it is not known whether curcumin affects oxidative stress after downhill running-induced muscle damage. Therefore, the purpose of this study was to investigate the effects of curcumin on oxidative stress following downhill running induced-muscle damage. We also investigated whether curcumin affects macrophage infiltration via chemokines such as MCP-1 and CXCL14. Male C57BL/6 mice were divided into four groups; rest, rest plus curcumin, downhill running, or downhill running plus curcumin. Downhill running mice ran at 22. m/min, -15% grade on the treadmill for 150. min. Curcumin (3. mg) was administered in oral administration immediately after downhill running. Hydrogen peroxide concentration and NADPH-oxidase mRNA expression in the downhill running mice were significantly higher than those in the rest mice, but these variables were significantly attenuated by curcumin administration in downhill running mice. In addition, mRNA expression levels of MCP-1, CXCL14 and F4/80 reflecting presence of macrophages in the downhill running mice were significantly higher than those in the rest mice. However, MCP-1 and F4/80 mRNA expression levels were significantly attenuated by curcumin administration in downhill running mice. Curcumin may attenuate oxidative stress following downhill running-induced muscle damage.
AB - Downhill running causes muscle damage, and induces oxidative stress and inflammatory reaction. Recently, it is shown that curcumin possesses anti-oxidant and anti-inflammatory potentials. Interestingly, curcumin reduces inflammatory cytokine concentrations in skeletal muscle after downhill running of mice. However, it is not known whether curcumin affects oxidative stress after downhill running-induced muscle damage. Therefore, the purpose of this study was to investigate the effects of curcumin on oxidative stress following downhill running induced-muscle damage. We also investigated whether curcumin affects macrophage infiltration via chemokines such as MCP-1 and CXCL14. Male C57BL/6 mice were divided into four groups; rest, rest plus curcumin, downhill running, or downhill running plus curcumin. Downhill running mice ran at 22. m/min, -15% grade on the treadmill for 150. min. Curcumin (3. mg) was administered in oral administration immediately after downhill running. Hydrogen peroxide concentration and NADPH-oxidase mRNA expression in the downhill running mice were significantly higher than those in the rest mice, but these variables were significantly attenuated by curcumin administration in downhill running mice. In addition, mRNA expression levels of MCP-1, CXCL14 and F4/80 reflecting presence of macrophages in the downhill running mice were significantly higher than those in the rest mice. However, MCP-1 and F4/80 mRNA expression levels were significantly attenuated by curcumin administration in downhill running mice. Curcumin may attenuate oxidative stress following downhill running-induced muscle damage.
KW - Curcumin
KW - Downhill running
KW - Macrophage
KW - Oxidative stress
KW - Skeletal muscle
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U2 - 10.1016/j.bbrc.2013.10.119
DO - 10.1016/j.bbrc.2013.10.119
M3 - Article
C2 - 24184481
AN - SCOPUS:84888294034
VL - 441
SP - 573
EP - 578
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -