CXCR4 stimulates macropinocytosis: Implications for cellular uptake of arginine-rich cell-penetrating peptides and HIV

Gen Tanaka, Ikuhiko Nakase, Yasunori Fukuda, Ryo Masuda, Shinya Oishi, Kazuya Shimura, Yoshimasa Kawaguchi, Tomoka Takatani-Nakase, Ülo Langel, Astrid Gräslund, Katsuya Okawa, Masao Matsuoka, Nobutaka Fujii, Yasumaru Hatanaka, Shiroh Futaki

研究成果: Article査読

73 被引用数 (Scopus)

抄録

CXCR4 is a coreceptor of HIV-1 infection in host cells. Through a photocrosslinking study to identify receptors involved in internalization of oligoarginine cell-penetrating peptides (CPPs), we found that CXCR4 serves as a receptor that stimulates macropinocytic uptake of the arginine 12-mer peptide (R12) but not of the 8-mer. We also found that stimulating CXCR4 with its intrinsic ligands, stromal cell-derived factor 1α and HIV-1 envelope glycoprotein 120, induced macropinocytosis. R12 had activity to prevent viral infection for HIV-1IIIB, a subtype of HIV-1 that uses CXCR4 as a coreceptor for entry into susceptible cells, whereas the addition of a macropinocytosis inhibitor, dimethylamiloride, resulted in enhancement of viral infection. The present study shows that CXCR4 triggers macropinocytosis, which may have implications for the cellular uptake of oligoarginine CPPs and internalization of HIV.

本文言語English
ページ(範囲)1437-1446
ページ数10
ジャーナルChemistry and Biology
19
11
DOI
出版ステータスPublished - 2012 11 21
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬理学
  • 創薬
  • 臨床生化学

フィンガープリント

「CXCR4 stimulates macropinocytosis: Implications for cellular uptake of arginine-rich cell-penetrating peptides and HIV」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル