Cyclic Peptides for Efficient Detection of Collagen

Koh K. Takita, Kazunori K. Fujii, Tetsuya Kadonosono, Ryo Masuda, Takaki Koide

研究成果: Article

4 引用 (Scopus)

抜粋

We report here a new class of collagen-binding peptides, cyclic collagen-mimetic peptides (cCMPs), that efficiently hybridize with the triple-helix-forming portions of collagen. cCMPs are composed of two parallel collagen-like (Xaa-Yaa-Gly)n strands with both termini tethered by covalent linkages. Enzyme-linked immunosorbent assays and western blotting analysis showed that cCMPs exhibit more potent affinity toward collagen than reported collagen-binding peptides and can specifically detect different collagen polypeptides in a mixture of proteins. Collagen secreted from cultured cells was detected by confocal microscopy with fluorescein-labeled cCMP. The cCMP is also shown to detect sensitively folding intermediates in the endoplasmic reticulum, something that was difficult to visualize with conventional collagen detectors. Molecular-dynamics simulations suggested that a cCMP forms a more stably hybridized product than its single-chain counterpart; this could explain why cCMP has higher affinity toward denatured collagen. These results indicate the usefulness of cCMPs as tools for detecting denatured collagen.

元の言語English
ページ(範囲)1613-1617
ページ数5
ジャーナルChemBioChem
19
発行部数15
DOI
出版物ステータスPublished - 2018 8 6

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

フィンガープリント Cyclic Peptides for Efficient Detection of Collagen' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用