Cyclic Peptides for Efficient Detection of Collagen

Koh K. Takita, Kazunori K. Fujii, Tetsuya Kadonosono, Ryo Masuda, Takaki Koide*

*この研究の対応する著者

研究成果: Article査読

11 被引用数 (Scopus)

抄録

We report here a new class of collagen-binding peptides, cyclic collagen-mimetic peptides (cCMPs), that efficiently hybridize with the triple-helix-forming portions of collagen. cCMPs are composed of two parallel collagen-like (Xaa-Yaa-Gly)n strands with both termini tethered by covalent linkages. Enzyme-linked immunosorbent assays and western blotting analysis showed that cCMPs exhibit more potent affinity toward collagen than reported collagen-binding peptides and can specifically detect different collagen polypeptides in a mixture of proteins. Collagen secreted from cultured cells was detected by confocal microscopy with fluorescein-labeled cCMP. The cCMP is also shown to detect sensitively folding intermediates in the endoplasmic reticulum, something that was difficult to visualize with conventional collagen detectors. Molecular-dynamics simulations suggested that a cCMP forms a more stably hybridized product than its single-chain counterpart; this could explain why cCMP has higher affinity toward denatured collagen. These results indicate the usefulness of cCMPs as tools for detecting denatured collagen.

本文言語English
ページ(範囲)1613-1617
ページ数5
ジャーナルChemBioChem
19
15
DOI
出版ステータスPublished - 2018 8 6

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 有機化学

フィンガープリント

「Cyclic Peptides for Efficient Detection of Collagen」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル