Cytosolic 20 alpha-hydroxysteroid dehydrogenase activity in spontaneous neoplasms in the dog and cat.

K. Shiota*, N. Sasaki, N. Hattori, R. Miura, T. Hasegawa, J. X. Guang, K. Noda, M. Takahashi

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Steroid-producing tissues such as ovary, placenta, testis and adrenal gland and non-steroid-producing cells including lymphocytes are known to contain 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD). In the present study, we measured and partially characterized the 20 alpha-HSD in 11 neoplastic tissues surgically removed from dogs and a cat. The tissues were pathologically classified as 4 benign and 7 malignant. The enzyme activities in the cytoplasmic fraction were positive in 6 of the 7 malignant tissues and 2 of the 4 benign ones. Following DEAE chromatography analysis of 2 malignant tumour samples, multiple forms of enzyme activities with different electric charges were detected. Furthermore, 20 alpha-HSD activity in the cytosol fraction from malignant tumours was increased dramatically by passage through the DEAE column, suggesting that the enzyme is present in the cytosol as an inactive or sequestered form.

本文言語English
ページ(範囲)549-552
ページ数4
ジャーナルThe Journal of veterinary medical science / the Japanese Society of Veterinary Science
53
4
出版ステータスPublished - 1991 8月
外部発表はい

ASJC Scopus subject areas

  • マーケティング
  • 獣医学(全般)

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