Daily expression of mRNAs for the mammalian clock genes Per2 and Clock in mouse suprachiasmatic nuclei and liver and human peripheral blood mononuclear cells

Miyako Takata, Naoto Burioka, Shigehiro Ohdo, Hiroshi Takane, Hideyuki Terazono, Masanori Miyata, Takanori Sako, Hisashi Suyama, Yasushi Fukuoka, Katsuyuki Tomita, Eiji Shimizu

研究成果: Article査読

66 被引用数 (Scopus)

抄録

The mammalian circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and in most peripheral tissues. Clock genes drive the biological clock. However, circadian expression variations of the human clock genes are still unclear. In this study, we analyzed the daily variations of mPer2 and mClock mRNA expression in both the mouse SCN and liver to evaluate the central and peripheral alterations in the rodent clock genes. We also examined whether there are the daily variations of the clock genes hPer2 and hClock in human peripheral blood mononuclear cells (PBMCs). The daily variation of mClock and mPer2 mRNA expression in mouse SCN and liver were determined at ZT2, ZT6, ZT10, ZT14, ZT18 or ZT22. We isolated PBMCs from 9 healthy volunteers at 9:00 and 21:00 and examined the expression of hPer2 and hClock mRNA by RT-PCR analysis. The animals exhibited a robust daily rhythm in the RNA levels of mPer2 in the SCN and liver (P

本文言語English
ページ(範囲)263-269
ページ数7
ジャーナルJapanese Journal of Pharmacology
90
3
DOI
出版ステータスPublished - 2002 11 1
外部発表はい

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

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