Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri

Shuhei Ota, Mai Yoshihara, Tomokazu Yamazaki, Tsuyoshi Takeshita, Aiko Hirata, Mami Konomi, Kenshiro Oshima, Masahira Hattori, Katerina Bišová, Vilém Zachleder, Shigeyuki Kawano*

*この研究の対応する著者

研究成果: Article査読

41 被引用数 (Scopus)

抄録

Phosphorus is an essential element for life on earth and is also important for modern agriculture, which is dependent on inorganic fertilizers from phosphate rock. Polyphosphate is a biological polymer of phosphate residues, which is accumulated in organisms during the biological wastewater treatment process to enhance biological phosphorus removal. Here, we investigated the relationship between polyphosphate accumulation and electron-dense bodies in the green alga Parachlorella kessleri. Under sulfur-depleted conditions, in which some symporter genes were upregulated, while others were downregulated, total phosphate accumulation increased in the early stage of culture compared to that under sulfur-replete conditions. The P signal was detected only in dense bodies by energy dispersive X-ray analysis. Transmission electron microscopy revealed marked ultrastructural variations in dense bodies with and without polyphosphate. Our findings suggest that the dense body is a site of polyphosphate accumulation, and P. kessleri has potential as a phosphate-accumulating organism.

本文言語English
論文番号25731
ジャーナルScientific Reports
6
DOI
出版ステータスPublished - 2016 5月 16
外部発表はい

ASJC Scopus subject areas

  • 一般

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