Deep sequencing reveals specific bacterial signatures in the subgingival microbiota of healthy subjects

Eri Ikeda, Takahiko Shiba, Yuichi Ikeda, Wataru Suda, Akinori Nakasato, Yasuo Takeuchi, Miyuki Azuma, Masahira Hattori, Yuichi Izumi

    研究成果: Article

    1 引用 (Scopus)

    抄録

    Objectives: This study aimed to define the comprehensive bacterial flora of the healthy oral cavity by identifying and comparing bacterial species in different subgingival sites using 454 sequencing of 16S rRNA genes. Materials and methods: Subgingival plaque samples were taken from six target teeth (central incisor, first premolar, and first molar in both the maxilla and mandible) of 10 periodontally healthy patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed. Results: Bacterial composition in phylum level was similar for all sites within the same individual irrespective of tooth location. Unweighted UniFrac distance values of microbiome also showed that average distance was significantly larger between subjects than between tooth locations of the same subjects. Conclusions: The present results clarify the lack of effect of tooth location in the healthy subgingival microbiota. Results may suggest that any subgingival site can demonstrate similar subject-specific microbiota. Clinical relevance: This investigation offers a better understanding of the uniqueness of the oral microbiome. The present study will facilitate sampling in future subgingival microbiological studies.

    元の言語English
    ジャーナルClinical Oral Investigations
    DOI
    出版物ステータスAccepted/In press - 2019 1 1

    Fingerprint

    High-Throughput Nucleotide Sequencing
    Microbiota
    Healthy Volunteers
    Tooth
    rRNA Genes
    Bacterial DNA
    Bicuspid
    Maxilla
    Incisor
    Mandible
    Mouth

    ASJC Scopus subject areas

    • Dentistry(all)

    これを引用

    Deep sequencing reveals specific bacterial signatures in the subgingival microbiota of healthy subjects. / Ikeda, Eri; Shiba, Takahiko; Ikeda, Yuichi; Suda, Wataru; Nakasato, Akinori; Takeuchi, Yasuo; Azuma, Miyuki; Hattori, Masahira; Izumi, Yuichi.

    :: Clinical Oral Investigations, 01.01.2019.

    研究成果: Article

    Ikeda, Eri ; Shiba, Takahiko ; Ikeda, Yuichi ; Suda, Wataru ; Nakasato, Akinori ; Takeuchi, Yasuo ; Azuma, Miyuki ; Hattori, Masahira ; Izumi, Yuichi. / Deep sequencing reveals specific bacterial signatures in the subgingival microbiota of healthy subjects. :: Clinical Oral Investigations. 2019.
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    abstract = "Objectives: This study aimed to define the comprehensive bacterial flora of the healthy oral cavity by identifying and comparing bacterial species in different subgingival sites using 454 sequencing of 16S rRNA genes. Materials and methods: Subgingival plaque samples were taken from six target teeth (central incisor, first premolar, and first molar in both the maxilla and mandible) of 10 periodontally healthy patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed. Results: Bacterial composition in phylum level was similar for all sites within the same individual irrespective of tooth location. Unweighted UniFrac distance values of microbiome also showed that average distance was significantly larger between subjects than between tooth locations of the same subjects. Conclusions: The present results clarify the lack of effect of tooth location in the healthy subgingival microbiota. Results may suggest that any subgingival site can demonstrate similar subject-specific microbiota. Clinical relevance: This investigation offers a better understanding of the uniqueness of the oral microbiome. The present study will facilitate sampling in future subgingival microbiological studies.",
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    AU - Ikeda, Eri

    AU - Shiba, Takahiko

    AU - Ikeda, Yuichi

    AU - Suda, Wataru

    AU - Nakasato, Akinori

    AU - Takeuchi, Yasuo

    AU - Azuma, Miyuki

    AU - Hattori, Masahira

    AU - Izumi, Yuichi

    PY - 2019/1/1

    Y1 - 2019/1/1

    N2 - Objectives: This study aimed to define the comprehensive bacterial flora of the healthy oral cavity by identifying and comparing bacterial species in different subgingival sites using 454 sequencing of 16S rRNA genes. Materials and methods: Subgingival plaque samples were taken from six target teeth (central incisor, first premolar, and first molar in both the maxilla and mandible) of 10 periodontally healthy patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed. Results: Bacterial composition in phylum level was similar for all sites within the same individual irrespective of tooth location. Unweighted UniFrac distance values of microbiome also showed that average distance was significantly larger between subjects than between tooth locations of the same subjects. Conclusions: The present results clarify the lack of effect of tooth location in the healthy subgingival microbiota. Results may suggest that any subgingival site can demonstrate similar subject-specific microbiota. Clinical relevance: This investigation offers a better understanding of the uniqueness of the oral microbiome. The present study will facilitate sampling in future subgingival microbiological studies.

    AB - Objectives: This study aimed to define the comprehensive bacterial flora of the healthy oral cavity by identifying and comparing bacterial species in different subgingival sites using 454 sequencing of 16S rRNA genes. Materials and methods: Subgingival plaque samples were taken from six target teeth (central incisor, first premolar, and first molar in both the maxilla and mandible) of 10 periodontally healthy patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed. Results: Bacterial composition in phylum level was similar for all sites within the same individual irrespective of tooth location. Unweighted UniFrac distance values of microbiome also showed that average distance was significantly larger between subjects than between tooth locations of the same subjects. Conclusions: The present results clarify the lack of effect of tooth location in the healthy subgingival microbiota. Results may suggest that any subgingival site can demonstrate similar subject-specific microbiota. Clinical relevance: This investigation offers a better understanding of the uniqueness of the oral microbiome. The present study will facilitate sampling in future subgingival microbiological studies.

    KW - 16S rRNA

    KW - Bacteria

    KW - Dental plaque

    KW - Microbiota

    KW - Tooth

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