Defensive bacteriome symbiont with a drastically reduced genome

Atsushi Nakabachi, Reiko Ueoka, Kenshiro Oshima, Roberta Teta, Alfonso Mangoni, Mihaela Gurgui, Neil J. Oldham, Gerhild Van Echten-Deckert, Keiko Okamura, Kohei Yamamoto, Hiromitsu Inoue, Moriya Ohkuma, Yuichi Hongoh, Shin Ya Miyagishima, Masahira Hattori, Jörn Piel, Takema Fukatsu

研究成果: Article査読

164 被引用数 (Scopus)

抄録

Diverse insect species harbor symbiotic bacteria, which play important roles such as provisioning nutrients and providing defense against natural enemies [1-6]. Whereas nutritional symbioses are often indispensable for both partners, defensive symbioses tend to be of a facultative nature [1-12]. The Asian citrus psyllid Diaphorina citri is a notorious agricultural pest that transmits Liberibacter spp. (Alphaproteobacteria), causing the devastating citrus greening disease or Huanglongbing [13, 14]. In a symbiotic organ called the bacteriome, D. citri harbors two distinct intracellular symbionts: a putative nutrition provider, Carsonella-DC (Gammaproteobacteria), and an unnamed betaproteobacterium with unknown function [15], for which we propose the name "Candidatus Profftella armatura." Here we report that Profftella is a defensive symbiont presumably of an obligate nature with an extremely streamlined genome. The genomes of Profftella and Carsonella-DC were drastically reduced to 464,857 bp and 174,014 bp, respectively, suggesting their ancient and mutually indispensible association with the host. Strikingly, 15% of the small Profftella genome encoded horizontally acquired genes for synthesizing a novel polyketide toxin. The toxin was extracted, pharmacologically and structurally characterized, and designated diaphorin. The presence of Profftella and its diaphorin-biosynthetic genes was perfectly conserved in the world's D. citri populations.

本文言語English
ページ(範囲)1478-1484
ページ数7
ジャーナルCurrent Biology
23
15
DOI
出版ステータスPublished - 2013 8 5
外部発表はい

ASJC Scopus subject areas

  • 農業および生物科学(全般)
  • 生化学、遺伝学、分子生物学(全般)

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