Deformability-based microfluidic microdroplet sorting as a screening method for single agarolytic bacterial cells

Mikihisa Muta, Kai Saito, Ryo Iizuka*, Wataru Kawakubo, Dong Hyun Yoon, Mei Ito, Yuji Hatada, Tetsushi Sekiguchi, Shuichi Shoji, Takashi Funatsu

*この研究の対応する著者

研究成果: Conference contribution

抄録

We have developed a deformability-based microfluidic microdroplet method for screening microbial cells secreting macromolecule-degrading enzymes. In this method, microbial cells are encapsulated as single cells in water-in-oil (W/O) microdroplets with macromolecule, whose shape becomes deformable as the macromolecule is progressively degraded into smaller molecules. Screening is achieved using a microfluidic device that passively sorts the deformed W/O microdroplets. Using this method, we successfully sorted agarose-containing microdroplets encapsulating single bacterial cells that hydrolyze agarose.

本文言語English
ホスト出版物のタイトルMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ48-49
ページ数2
ISBN(電子版)9781733419017
出版ステータスPublished - 2020
イベント24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
継続期間: 2020 10 42020 10 9

出版物シリーズ

名前MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period20/10/420/10/9

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング
  • 化学 (全般)
  • 制御およびシステム工学

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