Degree of dissociation of apohemoglobin studied by nanosecond fluorescence-polarization technique

Kazuhiko Kinosita*, Shigeki Mitaku, Akira Ikegami

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

A fluorescent dye 1-anilino-8-naphthalene sulfonate was complexed with human apohemoglobin and sperm whale apomyoglobin. Nanosecond fluorescencepolarization kinetics were measured for each of these complexes in KCl solutions to obtain their fluorescence lifetimes and rotational correlation times. The rotational correlation time of apohemoglobin-dye complex was found to be 21 ns, which was about twice that of apomyoglobin-dye complex, 11 ns. These values were constant over an ionic strength range from 0 to 1.7. Circular dichroism spectra (215-300 nm) and fluorescence lifetimes of the complexes were also found to be independent of the ionic strength, indicating that no gross conformational change occurs with the change in the salt concentration. These results suggest that apohemoglobin remains dimeric over the ionic-strength range examined.

本文言語English
ページ(範囲)10-14
ページ数5
ジャーナルBBA - Protein Structure
393
1
DOI
出版ステータスPublished - 1975 5 30
外部発表はい

ASJC Scopus subject areas

  • 医学(全般)

フィンガープリント

「Degree of dissociation of apohemoglobin studied by nanosecond fluorescence-polarization technique」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル