Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system

Sachiko Yoshie, Naohiro Noda, Satoshi Tsuneda, Akira Hirata, Yuhei Inamori

    研究成果: Article

    12 引用 (Scopus)

    抄録

    Three 16S rRNA-targeted oligonucleotide probes, namely, PSMg437 targeting several members of the genus Pseudomonas, Hlm474 targeting several members of the genus Halomonas, and Clw844 targeting several members of the genus Colwellia, were designed. The microbial community structure and nitrogen removal ability of nitrate-containing saline wastewater treatment systems with anaerobic packed bed and fluidized bed were monitored. Direct cell counting using fluorescence in situ hybridization (FISH) images revealed that various phylogenetic groups were evenly distributed in the anaerobic packed bed whereas members of the genus Halomonas were dominant particularly in the anaerobic fluidized bed. These results suggest that the microbial communities produced by different flow conditions correlated with denitrification ability in saline industrial wastewater treatment systems.

    元の言語English
    ページ(範囲)183-189
    ページ数7
    ジャーナルFEMS Microbiology Letters
    235
    発行部数1
    DOI
    出版物ステータスPublished - 2004 6 1

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    Halomonas
    Denitrification
    Oligonucleotide Probes
    Waste Water
    Alteromonadaceae
    Pseudomonas
    Fluorescence In Situ Hybridization
    Nitrates
    Nitrogen

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Applied Microbiology and Biotechnology
    • Microbiology

    これを引用

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    AU - Yoshie, Sachiko

    AU - Noda, Naohiro

    AU - Tsuneda, Satoshi

    AU - Hirata, Akira

    AU - Inamori, Yuhei

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