抄録
A novel fluoroimmunoassay method using bacterial magnetic particles for the highly sensitive detection of bacteria has been developed. Fluorescein isothiocyanate (FITC) conjugated monoclonal anti-Escherichia coli antibody was immobilized onto bacterial magnetic particles (BMPs) using a heterobifunctional reagent, N-succinimdyl 3-(2-pyridyldithio)propionate (SPDP). E. coli cells were reacted with FITC-antibody-BMP conjugates for 15 min in an inhomogeneous magnetic field which enhanced aggregation. The cell/BMP complexes sedimented, causing relative fluorescence intensity of the solution to decrease with increasing microbial cell concentration. A linear relationship was obtained between the relative fluorescence intensity and cell concentration in the range of 102-106 cells/mL. Selectivity of this detection system was satisfactory. Monoclonal antibody immobilized on BMPs was also applied to the specific removal of E, coli from the bacterial suspension.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 2036-2039 |
| ページ数 | 4 |
| ジャーナル | Analytical Chemistry |
| 巻 | 65 |
| 発行部数 | 15 |
| DOI | |
| 出版物ステータス | Published - 1993 8 1 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
これを引用
Detection and Removal of Escherichia coli Using Fluorescein Isothiocyanate Conjugated Monoclonal Antibody Immobilized on Bacterial Magnetic Particles. / Nakamura, Noriyuki; Burgess, J. Grant; Yagiuda, Kaoru; Kudo, Satoko; Sakaguchi, Toshifumi; Matsunaga, Tadashi.
：: Analytical Chemistry, 巻 65, 番号 15, 01.08.1993, p. 2036-2039.研究成果: Article
}
TY - JOUR
T1 - Detection and Removal of Escherichia coli Using Fluorescein Isothiocyanate Conjugated Monoclonal Antibody Immobilized on Bacterial Magnetic Particles
AU - Nakamura, Noriyuki
AU - Burgess, J. Grant
AU - Yagiuda, Kaoru
AU - Kudo, Satoko
AU - Sakaguchi, Toshifumi
AU - Matsunaga, Tadashi
PY - 1993/8/1
Y1 - 1993/8/1
N2 - A novel fluoroimmunoassay method using bacterial magnetic particles for the highly sensitive detection of bacteria has been developed. Fluorescein isothiocyanate (FITC) conjugated monoclonal anti-Escherichia coli antibody was immobilized onto bacterial magnetic particles (BMPs) using a heterobifunctional reagent, N-succinimdyl 3-(2-pyridyldithio)propionate (SPDP). E. coli cells were reacted with FITC-antibody-BMP conjugates for 15 min in an inhomogeneous magnetic field which enhanced aggregation. The cell/BMP complexes sedimented, causing relative fluorescence intensity of the solution to decrease with increasing microbial cell concentration. A linear relationship was obtained between the relative fluorescence intensity and cell concentration in the range of 102-106 cells/mL. Selectivity of this detection system was satisfactory. Monoclonal antibody immobilized on BMPs was also applied to the specific removal of E, coli from the bacterial suspension.
AB - A novel fluoroimmunoassay method using bacterial magnetic particles for the highly sensitive detection of bacteria has been developed. Fluorescein isothiocyanate (FITC) conjugated monoclonal anti-Escherichia coli antibody was immobilized onto bacterial magnetic particles (BMPs) using a heterobifunctional reagent, N-succinimdyl 3-(2-pyridyldithio)propionate (SPDP). E. coli cells were reacted with FITC-antibody-BMP conjugates for 15 min in an inhomogeneous magnetic field which enhanced aggregation. The cell/BMP complexes sedimented, causing relative fluorescence intensity of the solution to decrease with increasing microbial cell concentration. A linear relationship was obtained between the relative fluorescence intensity and cell concentration in the range of 102-106 cells/mL. Selectivity of this detection system was satisfactory. Monoclonal antibody immobilized on BMPs was also applied to the specific removal of E, coli from the bacterial suspension.
UR - http://www.scopus.com/inward/record.url?scp=0027647935&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027647935&partnerID=8YFLogxK
U2 - 10.1021/ac00063a018
DO - 10.1021/ac00063a018
M3 - Article
C2 - 8372968
AN - SCOPUS:0027647935
VL - 65
SP - 2036
EP - 2039
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 15
ER -