Detection of biomolecular interaction between biotin and streptavidin on a self-assembled monolayer using magnetic nanoparticles

Atsushi Arakaki, Sho Hideshima, Takahito Nakagawa, Daisuke Niwa, Tsuyoshi Tanaka, Tadashi Matsunaga, Tetsuya Osaka*

*この研究の対応する著者

研究成果査読

40 被引用数 (Scopus)

抄録

For developing a magnetic bioassay system, an investigation to determine the presence of a specific biomolecular interaction between biotin and streptavidin was done using magnetic nanoparticles and a silicon substrate with a self-assembled monolayer. Streptavidin was immobilized on the magnetic particles, and biotin was attached to the monolayer-modified substrate. The reaction of streptavidin-modified magnetic particles on the biotin-modified substrate was clearly observed under an optical microscope. The magnetic signals from the particles were detected using a magnetic force microscope. The results of this study demonstrate that the combination of a monolayer-modified substrate with biomolecule-modified magnetic particles is useful for detecting biomolecular interactions in medical and diagnostic analyses.

本文言語English
ページ(範囲)543-546
ページ数4
ジャーナルBiotechnology and bioengineering
88
4
DOI
出版ステータスPublished - 2004 11 20

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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