Detection of Microbial Cells by Cyclic Voltammetry

Tadashi Matsunaga*, Yoichi Namba

*この研究の対応する著者

研究成果: Article査読

71 被引用数 (Scopus)

抄録

A novel method for detecting microbial cells has been developed by applying cyclic voitammetry to a cell suspension of S. cerevlslae. A basal plane pyrolytic graphite electrode was employed as a working electrode. An anodic peak current appeared at 0.74 V vs. SSCE. A linear relationship was obtained between the peak current and the cell concentration in the range (0.1-1.9) × 108 cells·mL-1. The peak currents were reproducible with an average relative error of 4%. The peak currents per 108 cells were almost constant for cells harvested at an exponential growth period and a stational growth period. The peak current was not obtained from protoplasts of S. cerevlslae. It was shown that CoA existing in the cell wall mediated an electron transfer between the cells and the graphite electrode.

本文言語English
ページ(範囲)798-801
ページ数4
ジャーナルAnalytical Chemistry
56
4
DOI
出版ステータスPublished - 1984
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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