Detection of Penicillin G Produced by Penicillium chrysogenum with Raman Microspectroscopy and Multivariate Curve Resolution-Alternating Least-Squares Methods

Shumpei Horii, Masahiro Ando, Ashok Zachariah Samuel, Akira Take, Takuji Nakashima, Atsuko Matsumoto, Yō Ko Takahashi, Haruko Takeyama*

*この研究の対応する著者

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Raman microspectroscopy is a minimally invasive technique that can identify molecules without labeling. In this study, we demonstrate the detection of penicillin G inside Penicillium chrysogenum KF425 fungal cells. Raman spectra acquired from the fungal cells had highly overlapped spectroscopic signatures and hence were analyzed with multivariate curve resolution by alternating least-squares (MCR-ALS) to extract the spectra of individual molecular constituents. In addition to detecting spatial distribution of multiple constituents such as proteins and lipids inside the fungal body, we could also observe the subcellular localization of penicillin G. This methodology has the potential to be employed in screening the production of bioactive compounds by microorganisms.

本文言語English
ページ(範囲)3223-3229
ページ数7
ジャーナルJournal of Natural Products
83
11
DOI
出版ステータスPublished - 2020 11 25

ASJC Scopus subject areas

  • 分析化学
  • 分子医療
  • 薬理学
  • 薬科学
  • 創薬
  • 補完代替医療
  • 有機化学

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