Detection of tumor marker in blood serum using antibody-modified field effect transistor with optimized BSA blocking

Sho Hideshima, Ryosuke Sato, Sayaka Inoue, Shigeki Kuroiwa, Tetsuya Osaka*

*この研究の対応する著者

研究成果査読

53 被引用数 (Scopus)

抄録

This paper demonstrates that antibody-modified field effect transistors (FETs), which were treated with bovine serum albumin (BSA) blocking, had quantitative capability for tumor marker detection in blood serum. Antibody-modified surface covered with 0.1% BSA effectively minimized nonspecific adsorption of blood proteins. Tumor markers with their isoelectric points of around 5 generated strong signals in body environmental pH (pH 7.4) in proteins with isoelectric points ranging from 3 to 10. Detection of a tumor marker alpha-fetoprotein in blood serum at required level for clinical diagnosis (10 ng/mL) was achieved. The obtained results suggest that the fabricated FETs be expected to practical application in medical fields for direct detection of multi-tumor markers from blood.

本文言語English
ページ(範囲)146-150
ページ数5
ジャーナルSensors and Actuators, B: Chemical
161
1
DOI
出版ステータスPublished - 2012 1 3

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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