Determination of cholesterol with a laboratory-built chemiluminescence system

Akiyoshi Taniguchi, Yuzuru Hayashi, Hidetaka Yuki

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.

本文言語English
ページ(範囲)95-100
ページ数6
ジャーナルAnalytica Chimica Acta
188
C
DOI
出版ステータスPublished - 1986
外部発表はい

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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