TY - JOUR
T1 - Determination of cholesterol with a laboratory-built chemiluminescence system
AU - Taniguchi, Akiyoshi
AU - Hayashi, Yuzuru
AU - Yuki, Hidetaka
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1986
Y1 - 1986
N2 - Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.
AB - Chemiluminescence measurement by a laboratory-built computer system equipped with an automated injection device is applied to a cholesterol enzymatic assay. The hydrogen peroxide produced by cholesterol esterase and oxidase is mixed rapidly with horseradish peroxidase-luminol solution (pH 10.5) and the light emission data are processed by a Fourier-transform digital filter to produce peak-height measurements. A blood serum volume of 0.2 μl and an observation time of 0.5 s are required.
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U2 - 10.1016/S0003-2670(00)86033-X
DO - 10.1016/S0003-2670(00)86033-X
M3 - Article
AN - SCOPUS:0002333727
VL - 188
SP - 95
EP - 100
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - C
ER -