Determination of trace levels of fatty acid metal salts by high-performance liquid chromatography with fluorescence prelabeling

Katsumi Hayashi, Jiro Kawase, Koichi Yoshimura, Katsutoshi Ara, Kazuro Tsuji

研究成果: Article査読

32 被引用数 (Scopus)

抄録

A simple method is described for picomole determinations of fatty acid metal salts. Fatty acid salts are directly labeled with 4-bromomethyl-7-methoxycoumarin in the presence of excess ethylenediaminetetraacetic acid tripotassium salt without any solvent extractions. The fluorescence derivatives of fatty acids are separated by reverse-phase high-performance liquid chromatography followed by fluorometric detection. The response of each fatty acid (C8-C18) calcium salt is linear from 1 to 50 μg/ml of samples. The detection limit is about 7 pmol. Good recoveries are obtained for the calcium salts of myrystic acid and soap (C8-C18, C18:1,2). The new method is successfully applied to the study on biodegradation of fatty acids in river water.

本文言語English
ページ(範囲)314-320
ページ数7
ジャーナルAnalytical Biochemistry
136
2
DOI
出版ステータスPublished - 1984 2 1
外部発表はい

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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