Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles

Tsuyoshi Tanaka, Kohei Maruyama, Kiyoushi Yoda, Etsuo Nemoto, Yuuji Udagawa, Hideki Nakayama, Haruko Takeyama, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

27 被引用数 (Scopus)

抄録

We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to double-stranded DNA. The workstation is equipped with a 96-way automated pipetter which collects and dispenses fluids as it moves in x- and z-directions. The platform contains a disposable tip rack station, a reagent vessel serving as a stock for POPO-3 and FITC-labeled probes and a reaction station for a 96-well microtiter plate. BMPs were collected by attaching a neodymium iron boron sintered (Nd-Fe-B) magnet on the bottom of the microtiter plate. This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs.

本文言語English
ページ(範囲)325-330
ページ数6
ジャーナルBiosensors and Bioelectronics
19
4
DOI
出版ステータスPublished - 2003 12月 15
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生物理学
  • 生体医工学
  • 電気化学

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