Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles

Tsuyoshi Tanaka, Kohei Maruyama, Kiyoushi Yoda, Etsuo Nemoto, Yuuji Udagawa, Hideki Nakayama, Haruko Takeyama, Tadashi Matsunaga

研究成果: Article

23 引用 (Scopus)

抄録

We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to double-stranded DNA. The workstation is equipped with a 96-way automated pipetter which collects and dispenses fluids as it moves in x- and z-directions. The platform contains a disposable tip rack station, a reagent vessel serving as a stock for POPO-3 and FITC-labeled probes and a reaction station for a 96-well microtiter plate. BMPs were collected by attaching a neodymium iron boron sintered (Nd-Fe-B) magnet on the bottom of the microtiter plate. This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs.

元の言語English
ページ(範囲)325-330
ページ数6
ジャーナルBiosensors and Bioelectronics
19
発行部数4
DOI
出版物ステータスPublished - 2003 12 15
外部発表Yes

Fingerprint

Fluorescein-5-isothiocyanate
Nucleotides
Polymorphism
Single Nucleotide Polymorphism
Magnetospirillum
DNA
Genotype
Neodymium
Magnetic separation
Fluorescence Resonance Energy Transfer
Boron
Magnets
Heating
Assays
Iron
Fluorescence
Fluids
POPO 3
Direction compound

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

これを引用

Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles. / Tanaka, Tsuyoshi; Maruyama, Kohei; Yoda, Kiyoushi; Nemoto, Etsuo; Udagawa, Yuuji; Nakayama, Hideki; Takeyama, Haruko; Matsunaga, Tadashi.

:: Biosensors and Bioelectronics, 巻 19, 番号 4, 15.12.2003, p. 325-330.

研究成果: Article

Tanaka, Tsuyoshi ; Maruyama, Kohei ; Yoda, Kiyoushi ; Nemoto, Etsuo ; Udagawa, Yuuji ; Nakayama, Hideki ; Takeyama, Haruko ; Matsunaga, Tadashi. / Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles. :: Biosensors and Bioelectronics. 2003 ; 巻 19, 番号 4. pp. 325-330.
@article{9d4ef45d06644051bc0d302a91ed7210,
title = "Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles",
abstract = "We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to double-stranded DNA. The workstation is equipped with a 96-way automated pipetter which collects and dispenses fluids as it moves in x- and z-directions. The platform contains a disposable tip rack station, a reagent vessel serving as a stock for POPO-3 and FITC-labeled probes and a reaction station for a 96-well microtiter plate. BMPs were collected by attaching a neodymium iron boron sintered (Nd-Fe-B) magnet on the bottom of the microtiter plate. This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs.",
keywords = "Aldehyde dehydrogenase 2 gene, Bacterial magnetic particles (BMPs), Fluorescence resonance energy transfer, Single nucleotide polymorphism (SNP)",
author = "Tsuyoshi Tanaka and Kohei Maruyama and Kiyoushi Yoda and Etsuo Nemoto and Yuuji Udagawa and Hideki Nakayama and Haruko Takeyama and Tadashi Matsunaga",
year = "2003",
month = "12",
day = "15",
doi = "10.1016/S0956-5663(03)00189-1",
language = "English",
volume = "19",
pages = "325--330",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles

AU - Tanaka, Tsuyoshi

AU - Maruyama, Kohei

AU - Yoda, Kiyoushi

AU - Nemoto, Etsuo

AU - Udagawa, Yuuji

AU - Nakayama, Hideki

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

PY - 2003/12/15

Y1 - 2003/12/15

N2 - We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to double-stranded DNA. The workstation is equipped with a 96-way automated pipetter which collects and dispenses fluids as it moves in x- and z-directions. The platform contains a disposable tip rack station, a reagent vessel serving as a stock for POPO-3 and FITC-labeled probes and a reaction station for a 96-well microtiter plate. BMPs were collected by attaching a neodymium iron boron sintered (Nd-Fe-B) magnet on the bottom of the microtiter plate. This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs.

AB - We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to double-stranded DNA. The workstation is equipped with a 96-way automated pipetter which collects and dispenses fluids as it moves in x- and z-directions. The platform contains a disposable tip rack station, a reagent vessel serving as a stock for POPO-3 and FITC-labeled probes and a reaction station for a 96-well microtiter plate. BMPs were collected by attaching a neodymium iron boron sintered (Nd-Fe-B) magnet on the bottom of the microtiter plate. This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs.

KW - Aldehyde dehydrogenase 2 gene

KW - Bacterial magnetic particles (BMPs)

KW - Fluorescence resonance energy transfer

KW - Single nucleotide polymorphism (SNP)

UR - http://www.scopus.com/inward/record.url?scp=0242570444&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242570444&partnerID=8YFLogxK

U2 - 10.1016/S0956-5663(03)00189-1

DO - 10.1016/S0956-5663(03)00189-1

M3 - Article

C2 - 14615090

AN - SCOPUS:0242570444

VL - 19

SP - 325

EP - 330

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 4

ER -