Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles

Tsuyoshi Tanaka, Kohei Maruyama, Kiyoushi Yoda, Etsuo Nemoto, Yuuji Udagawa, Hideki Nakayama, Haruko Takeyama, Tadashi Matsunaga

研究成果: Article

24 引用 (Scopus)

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We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to double-stranded DNA. The workstation is equipped with a 96-way automated pipetter which collects and dispenses fluids as it moves in x- and z-directions. The platform contains a disposable tip rack station, a reagent vessel serving as a stock for POPO-3 and FITC-labeled probes and a reaction station for a 96-well microtiter plate. BMPs were collected by attaching a neodymium iron boron sintered (Nd-Fe-B) magnet on the bottom of the microtiter plate. This system permits the simultaneous heating and magnetic separation of 96 samples per assay. The genotypes ALDH2*1 and ALDH2*2 were discriminated by calculating the relative fluorescence intensities on BMPs.

元の言語English
ページ(範囲)325-330
ページ数6
ジャーナルBiosensors and Bioelectronics
19
発行部数4
DOI
出版物ステータスPublished - 2003 12 15

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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