Development of a cell surface display system in a magnetotactic bacterium, "Magnetospirillum magneticum" AMB-1

Masayoshi Tanaka, Yuko Nakata, Tetsushi Mori, Yoshiko Okamura, Hitoshi Miyasaka, Haruko Takeyama, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

21 被引用数 (Scopus)

抄録

Bacterial cell surface display is a widely used technology for bioadsorption and for the development of a variety of screening systems. Magnetotactic bacteria are unique species of bacteria due to the presence of magnetic nanoparticles within them. These intracellular, nanosized (50 to 100 nm) magnetic nanoparticles enable the cells to migrate and be manipulated by magnetic force. In this work, using this unique characteristic and based on whole-genomic and comprehensive proteomic analyses of these bacteria, a cell surface display system has been developed by expressing hexahistidine residues within the outer coiled loop of the membrane-specific protein (Msp1) of the "Magnetospirillum magneticum" (proposed name) AMB-1 bacterium. The optimal display site of the hexahistidine residues was successfully identified via secondary structure prediction, immunofluorescence microscopy, and heavy metal binding assay. The established AMB-1 transformant showed high immunofluorescence response, high Cd2+ binding, and high recovery efficiency in comparison to those of the negative control when manipulated by magnetic force.

本文言語English
ページ(範囲)3342-3348
ページ数7
ジャーナルApplied and Environmental Microbiology
74
11
DOI
出版ステータスPublished - 2008 6月
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 食品科学
  • 応用微生物学とバイオテクノロジー
  • 生態学

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