Development of a novel method for operating magnetic particles, magtration technology, and its use for automating nucleic acid purification

Kimimichi Obata, Osamu Segawa, Mitsuru Yakabe, Yoshikazu Ishida, Toshihiro Kuroita, Katsunori Ikeda, Bunsei Kawakami, Yoshihisa Kawamura, Masafumi Yohda, Tadashi Matsunaga, Hideji Tajima

研究成果: Article査読

55 被引用数 (Scopus)

抄録

Magnetic particles are useful for simple and efficient nucleic acid extraction. To achieve fully automated nucleic acid extraction and purification using magnetic particles, a new method for operating magnetic particles, Magtration Technology, was developed. In this method, magnetic separation is performed in a specially designed disposable tip. This enables high recovery of magnetic particles with high reproducibility. The features of this technology are (i) a simple mechanism for process control and (ii) flexible software to enable adaptation to commercially available reagents. Automated instruments based on Magtration Technology were developed and used for nucleic acid extraction. Total DNA, total RNA and plasmids were purified by Magtration Technology at an efficiency comparable to that of manual methods.

本文言語English
ページ(範囲)500-503
ページ数4
ジャーナルJournal of Bioscience and Bioengineering
91
5
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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