Development of a poly-dimethylsiloxane microfluidic device for single cell isolation and incubation

Yoshinori Yamaguchi*, Takahiro Arakawa, Naoya Takeda, Yoshikuni Edagawa, Shuichi Shoji

*この研究の対応する著者

研究成果: Article査読

30 被引用数 (Scopus)

抄録

Although single cell manipulation has been developed for precise understanding of cell biology, it is still difficult to handle single cells because of their morphology including the size variation and the flexibility. We developed a single cell manipulation device featuring microchannels and micropockets for single cell capture and cultivation. Single cells were captured noninvasively and sequentially, and each cell was repeatedly sub-cultured to four generations. The single cell manipulation devicewas microfabricated with two main parallel channels allowing the cell suspension and the carrier flow to be injected separately. Those channels, that are main channel and buffer channel, were connected with a narrow (3μm) drain channel, and single cell capture pockets were placed at the point where the main channels and the drain channel connected. A gentle flow was produced in the drain channel because of the difference in the flow rate between the main channel and buffer channel, realized the individual single cell isolation in the capture pocket. When a single cell was captured in a single cell pocket, the captured cell capped the drain channel and caused the other cells that were flowing through the channel to go to the next capture pockets. The ratio between the cells thatwere captured and the cells that passed through the main channel was about 70%. The captured singe cellwas cultured successively in the same pocket, and the cells divided into four cells. The doubling times of two cells that grew in the first cell division were slightly different (10 min). These fundamental single cell manipulations were carried out mainly by controlling the flow rate, essentially the pressure on each channel. Occasionally the manipulations also were carried out by changing the shapes and the sizes of the micropockets in the microfluidic device. Since this device was used successfully for the noninvasive isolation and long-term cultivation of single cells, it can contribute to various biological analyses, such as biopsy, noninvasive bioanalysis, and the morphology of single cells.

本文言語English
ページ(範囲)555-561
ページ数7
ジャーナルSensors and Actuators, B: Chemical
136
2
DOI
出版ステータスPublished - 2009

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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