Development of a rapid isolation procedure for coccolith ultrafine particles produced by coccolithophorid algae

Hiroyuki Takano, Eichi Manabe, Morio Hirano, Megumi Okazaki, J. Grant Burgess, Noriyuki Nakamura, Tadashi Matsunaga

研究成果: Article査読

11 被引用数 (Scopus)

抄録

A rapid procedure for effective purification of large quantities of coccolith ultrafine particles from marine algae is reported. Coccoliths are detached from cells by optimized sonication in the presence of 50 m M NaHCO3. Contaminating cell debris is then removed from coccoliths by cycles of washing and floatation. Coccolith particles were purified from Emiliania huxleyi and Pleurochrysis carterae. The surface area of these particles is three to five times greater than synthetic calcite particles. Glucose oxidase and uricase have been immobilized onto purified coccolith ultrafine particles to illustrate their potential as a support material for biotechnological application.

本文言語English
ページ(範囲)239-247
ページ数9
ジャーナルApplied Biochemistry and Biotechnology
39-40
1
DOI
出版ステータスPublished - 1993 9
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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