A rapid procedure for effective purification of large quantities of coccolith ultrafine particles from marine algae is reported. Coccoliths are detached from cells by optimized sonication in the presence of 50 m M NaHCO3. Contaminating cell debris is then removed from coccoliths by cycles of washing and floatation. Coccolith particles were purified from Emiliania huxleyi and Pleurochrysis carterae. The surface area of these particles is three to five times greater than synthetic calcite particles. Glucose oxidase and uricase have been immobilized onto purified coccolith ultrafine particles to illustrate their potential as a support material for biotechnological application.
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