A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
|ジャーナル||Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology|
|出版ステータス||Published - 2001 5 23|
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Molecular Biology