Development of high-sensitive cytotoxic detection system using HSP70B promoter

K. I. Wada*, L. Xu, T. Okano, A. Taniguchi

*この研究の対応する著者

研究成果: Conference contribution

抄録

The development of a high-sensitive cytotoxic detection system using heat-shock proteins (HSP)70B promoter was discussed. Human liver carcinoma cell line was grown in DMEM at 37 °C in a humidified atmosphere with 5% CO 2. Cell viability was evaluated by quantification of DNA performed by using Cyquant Proliferation Assay kit. Subsequent sequencing revealed that transcriptional starting site was mapped at - 110 bp from the translational starting site in HepG2. These results suggests that the effect of low concentration of cadmium chloride on cytotoxicity could be detected by luciferase analysis using a part of HASP70B promoter.

本文言語English
ホスト出版物のタイトルTransactions - 7th World Biomaterials Congress
ページ数1
出版ステータスPublished - 2004 12月 1
外部発表はい
イベントTransactions - 7th World Biomaterials Congress - Sydney, Australia
継続期間: 2004 5月 172004 5月 21

出版物シリーズ

名前Transactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
国/地域Australia
CitySydney
Period04/5/1704/5/21

ASJC Scopus subject areas

  • 工学(全般)

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