Development of rapid detection system for allergic reaction using rat basophilic leukemia (RBL-1) cells

Noriyuki Nakamura*, Shizuko Kumazawa, Koji Sode, Tadashi Matsunaga

*この研究の対応する著者

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We have investigated the electrochemical detection system of allergic reaction using rat basophilic leukemia (RBL-1) cells by applying cyclic voltammetry. When the potential of the electrode is run in the range 0-1.0 V versus a saturated calomel electrode (SCE), an anodic peak current appears around 0.34 V. This peak current or RBL-1 cells is attributed to intracellular serotonin. After the addition of allergen, this peak current increases with time and the maximum current (0.82 μA per 2×105 cells) is obtained after 20 min. On the other hand, the current is constant when the allergen is not added. This current increase is attributed to localization of the intracellular serotonin and is specific for allergen.

本文言語English
ページ(範囲)312-314
ページ数3
ジャーナルSensors and Actuators: B. Chemical
13
1-3
DOI
出版ステータスPublished - 1993
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 電気化学
  • 電子工学および電気工学

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