Development of the automated circulating tumor cell recovery system with microcavity array

Ryo Negishi, Masahito Hosokawa, Seita Nakamura, Hisashige Kanbara, Masafumi Kanetomo, Yoshihito Kikuhara, Tsuyoshi Tanaka, Tadashi Matsunaga, Tomoko Yoshino*

*この研究の対応する著者

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Circulating tumor cells (CTCs) are well recognized as useful biomarker for cancer diagnosis and potential target of drug discovery for metastatic cancer. Efficient and precise recovery of extremely low concentrations of CTCs from blood has been required to increase the detection sensitivity. Here, an automated system equipped with a microcavity array (MCA) was demonstrated for highly efficient and reproducible CTC recovery. The use of MCA allows selective recovery of cancer cells from whole blood on the basis of differences in size between tumor and blood cells. Intra- and inter-assays revealed that the automated system achieved high efficiency and reproducibility equal to the assay manually performed by well-trained operator. Under optimized assay workflow, the automated system allows efficient and precise cell recovery for non-small cell lung cancer cells spiked in whole blood. The automated CTC recovery system will contribute to high-throughput analysis in the further clinical studies on large cohort of cancer patients.

本文言語English
ページ(範囲)438-442
ページ数5
ジャーナルBiosensors and Bioelectronics
67
DOI
出版ステータスPublished - 2015 5月 5
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生体医工学
  • バイオテクノロジー
  • 電気化学

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