Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum

Etsuro Ito, Atsuo Miyazawa, Hiroshi Takagi, Tohru Yoshioka, Tetsuro Horikoshi, Keiji Yanagisawa, Takeshi Nakamura, Yoshihisa Kudo, Masato Umeda, Keizo Inoue, Katsuhiko Mikoshiba

研究成果: Article

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The postnatal development of calcium-mobilizing systems was studied by both microfluorometric imaging analysis of Ca2+ on living rat cerebellar slices and immunohistochemical labeling of phosphatidylinositol 4,5-bisphosphate (PIP2) and inositol 1,4,5-trisphosphate binding protein (IP3BP) in fixed rat cerebellum. Stimulation with quisqualate (QA) or N-methyl-d-aspartate (NMDA) enhanced the Ca2+ level only diffusely on postnatal day (PND) 3, but more discretely on PNDs 7 and 15. On PND 21, QA-induced responses were localized in the molecular layer especially, but not in the granular layer. By contrast, NMDA mobilized Ca2+ prominently in the granular layer, but only weakly in the molecular layer. Localized expression of PIP2 in the molecular layer paralleled QA-induced Ca2+ mobilization, but IP3BP was expressed more diffusely. The present study offers the first direct evidence that PIP2, but not IP3BP, is essential for QA-induced Ca2+ mobilization in the cerebellar cortex.

元の言語English
ページ(範囲)179-188
ページ数10
ジャーナルNeuroscience Research
11
発行部数3
DOI
出版物ステータスPublished - 1991 8

ASJC Scopus subject areas

  • Neuroscience(all)

フィンガープリント Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Ito, E., Miyazawa, A., Takagi, H., Yoshioka, T., Horikoshi, T., Yanagisawa, K., Nakamura, T., Kudo, Y., Umeda, M., Inoue, K., & Mikoshiba, K. (1991). Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum. Neuroscience Research, 11(3), 179-188. https://doi.org/10.1016/0168-0102(91)90041-V