Developmental assembly of calcium-mobilizing systems for excitatory amino acids in rat cerebellum

Etsuro Ito, Atsuo Miyazawa, Hiroshi Takagi, Tohru Yoshioka*, Tetsuro Horikoshi, Keiji Yanagisawa, Takeshi Nakamura, Yoshihisa Kudo, Masato Umeda, Keizo Inoue, Katsuhiko Mikoshiba



14 被引用数 (Scopus)


The postnatal development of calcium-mobilizing systems was studied by both microfluorometric imaging analysis of Ca2+ on living rat cerebellar slices and immunohistochemical labeling of phosphatidylinositol 4,5-bisphosphate (PIP2) and inositol 1,4,5-trisphosphate binding protein (IP3BP) in fixed rat cerebellum. Stimulation with quisqualate (QA) or N-methyl-d-aspartate (NMDA) enhanced the Ca2+ level only diffusely on postnatal day (PND) 3, but more discretely on PNDs 7 and 15. On PND 21, QA-induced responses were localized in the molecular layer especially, but not in the granular layer. By contrast, NMDA mobilized Ca2+ prominently in the granular layer, but only weakly in the molecular layer. Localized expression of PIP2 in the molecular layer paralleled QA-induced Ca2+ mobilization, but IP3BP was expressed more diffusely. The present study offers the first direct evidence that PIP2, but not IP3BP, is essential for QA-induced Ca2+ mobilization in the cerebellar cortex.

ジャーナルNeuroscience Research
出版ステータスPublished - 1991 8月

ASJC Scopus subject areas

  • 神経科学(全般)


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