Dielectrophoresis-Assisted SPRF illumination biosensor for selective detection of biological substances

Chiaki Kuroda, Yoshimichi Ohki, Makoto Fujimaki

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

Aiming at detecting biological substances accurately and speedy, we are developing a dielectrophoresis-Assisted surface plasmon resonance fluorescence (SPRF) illumination biosensor. A sensing chip of the sensor has Al layers, which are used for electrodes and SPR excitation layers. In this study, we used Escherichia coli (E. coli) stained with 4', 6-diamidino-2-phenylindole (DAPI) as a target substance. First, the structure of the sensing chip was optimized to excite SPR on the Al electrodes by 405-nm light. Then, an emulsion with DAPI-stained E. coli was dropped on the sensing chip and illuminated by a 405-nm laser via a prism. When ac voltages were applied to the Al electrodes, the number of bright spots, due to fluorescence from DAPI-stained E. coli, increased on the electrodes. This result indicates that E. coli, which has a lower permittivity than the emulsion, was collected onto the electrodes where the electric field strength is lowest by negative dielectrophoresis. Therefore, we have succeeded in collecting target biological substances on the surface of the sensing chip and detecting them.

本文言語English
ホスト出版物のタイトルCEIDP 2017 - IEEE Conference on Electrical Insulation and Dielectric Phenomenon
出版社Institute of Electrical and Electronics Engineers Inc.
ページ253-256
ページ数4
ISBN(電子版)9781538611944
DOI
出版ステータスPublished - 2018 1 12
イベント2017 IEEE Conference on Electrical Insulation and Dielectric Phenomenon, CEIDP 2017 - Texas, United States
継続期間: 2017 10 222017 10 25

出版物シリーズ

名前Annual Report - Conference on Electrical Insulation and Dielectric Phenomena, CEIDP
2017-October
ISSN(印刷版)0084-9162

Other

Other2017 IEEE Conference on Electrical Insulation and Dielectric Phenomenon, CEIDP 2017
国/地域United States
CityTexas
Period17/10/2217/10/25

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 電子工学および電気工学

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